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T13-1 [D7S2742, UTR-9783]
T13-1 [D7S2742, UTR-9783]
規(guī)格:
貨期:
編號(hào):B162711
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 T13-1 [D7S2742, UTR-9783]
商品貨號(hào) B162711
Designations T13-1 [D7S2742, UTR-9783]
GenBank Number

M28668

Species Homo sapiens, human
Depositors JR Riordan
Vector
Construct size (kb): 4.900000095367432
Insert
DNA: cDNA
Insert lengths(kb): 2.099999904632568
Tissue: colon carcinoma T84 cell line
Gene product: cystic fibrosis transmembrane conductance regulator [CF]
Alleles: A1, A1, A1, A1, A2, B1, B2, A1, A1, A2, B1, B2, A2, A2, A2, A2
Insert Size (kb) 2.100
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
This insert contains an internal EcoRI site. An EcoRI digest of the plasmid results in fragments of 1.7 and 0.4 kb from the insert.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.05, 1.5, 0.42; PstI--4.9; XbaI--4.9; BglII--4.9; HindIII--4.9.
Contains exon 4 plus flanking upstream and downstream introns.
References

Riordan JR, et al. Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science 245: 1066-1073, 1989. PubMed: 2475911

J R Riordan, personal communication

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