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<em>Macaca mulatta</em> polyomavirus 1
<em>Macaca mulatta</em> polyomavirus 1
規(guī)格:
貨期:
編號(hào):B162812
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) Macaca mulatta polyomavirus 1
商品貨號(hào) B162812
Classification Polyomaviridae, Betapolyomavirus
Deposited As Macaca mulatta polyomavirus 1
Agent Macaca mulatta polyomavirus 1
Strain 776 [776-2E (WT)]
Common Name Simian virus 40, SV40
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL per vial
Storage Conditions -70°C or colder
Images VR-1901 Images
Effect on Host
CPE, vacuolization, rounding, and sloughing
Recommended Host
CV-1 cells (ATCC® CCL-70™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 9-16 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.

Name of Depositor J Butel, Baylor College of Medicine
Source Isolated from an Adenovirus type 1 seed stock, 1960.
References

Sweet BH and Hilleman MR.  The vacuolating virus, S.V.40. Proc Soc Exp Biol Med 105:420-427, 1960. PubMed: 13774265

Lednicky J and Folk WR. Two synthetic Sp1-binding sites functionally substitute for the 21-base-pair repeat region to activate simian virus 40 growth in CV-1 cells. J Virol 66(11): 6379-6390, 1992. PubMed: 1328672

Stewart AR, et al.  Identification of a variable region at the carboxy terminus of SV40 large T-antigen. Virology 221(2):355-361, 1996. PubMed: 8661447

Forsman ZH, et al.  Phylogenetic analysis of polyomavirus simian virus 40 from monkeys and humans reveals genetic variation.  J Virol 78(17): 9306-9316, 2004.  PubMed: 15308725

Swain JL, et al.  Effects of route of inoculation and viral genetic variation on antibody responses to polyomavirus SV40 in Syrian golden hamsters. Comp Med 62(5):400-408, 2012. PubMed: 23114044

Zhang S, et al.  Viral microRNA effects on pathogenesis of polyomavirus SV40 infections in Syrian golden hamsters. PLoS Pathog 10(2):e1003912, 2004. PubMed: 24516384

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