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141PF11
141PF11
規(guī)格:
貨期:
編號:B163569
品牌:Mingzhoubio

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產(chǎn)品名稱 141PF11
商品貨號 B163569
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with purified human IgG1 (kappa light chain) myeloma protein.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human kappa light chain
Cellular Products
immunoglobulin; monoclonal antibody; against human kappa light chain
Comments
Animals were immunized with purified human IgG1 (kappa light chain) myeloma protein.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Isotype IgG1; kappa light chain
    Name of Depositor JD Capra
    Deposited As mouse (B cell); mouse (myeloma)
    梅經(jīng)理 17280875617 1438578920
    胡經(jīng)理 13345964880 2438244627
    周經(jīng)理 17757487661 1296385441
    于經(jīng)理 18067160830 2088210172
    沈經(jīng)理 19548299266 2662369050
    李經(jīng)理 13626845108 972239479
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