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2D4
2D4
規(guī)格:
貨期:
編號:B163678
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 2D4
商品貨號 B163678
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with a distinct steric portion of the carbohydrate moiety of asialo GM2, specifically the C6 hydroxyl bearing group of the terminal N-acetylgalactosamine residue.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against asialo GM2
Cellular Products
immunoglobulin; monoclonal antibody; against asialo GM2
Comments
Animals were immunized with asialo GM2 non-covalently adsorbed to naked Salmonella minnesota.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with a distinct steric portion of the carbohydrate moiety of asialo GM2, specifically the C6 hydroxyl bearing group of the terminal N-acetylgalactosamine residue.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium RPMI 1640 medium, 85%; fetal bovine serum, 15%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype IgM
Name of Depositor WW Young
Deposited As mouse (B cell); mouse (myeloma)
References

Young WW Jr., et al. Production of monoclonal antibodies specific for two distinct steric portions of the glycolipid ganglio-N-triosylceramide (Asialo GM2). J. Exp. Med. 150: 1008-1019, 1979. PubMed: 512581

Young WW J, Hakomori SI. Therapy of mouse lymphoma with monoclonal antibodies to glycolipid: selection of low antigenic variants in vivo. Science 211: 487-489, 1981. PubMed: 7455688

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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