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Animals were immunized with a synthetic polypeptide with the sequence LGGGQYGEVYEG(C) corresponding to peptides 248 to 259 of the abl oncogene (formerly 368 to 379 of the v-abl oncogene).

Spleen cells were fused with Sp2/0 myeloma cells.

immunoglobulin; monoclonal antibody; against a synthetic abl oncogene peptide

immunoglobulin; monoclonal antibody; against a synthetic abl oncogene peptide

Animals were immunized with a synthetic polypeptide with the sequence LGGGQYGEVYEG(C) corresponding to peptides 248 to 259 of the abl oncogene (formerly 368 to 379 of the v-abl oncogene).

Spleen cells were fused with Sp2/0 myeloma cells.

The monoclonal antibody does not cross react with the homologous regions of src, yes, mos, fes, mil, raf, fms, fgr, erb B, neu, HER, HIR, CDC28, or the catalytic or the regulatory subunit of bovine cyclic AMP dependent protein kinase in indirect ELISA.

The antibody does detect a 120 kd protein in ANN-1 cells when tested in an immunoblot test.

The IgG1 and IgG2b reactivities of the antibody with the ABL peptide (248 to 259) was confirmed by ELISA. IgG1 was strongly positive while IgG2b was only slightly positive.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.

Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

culture medium 95%; DMSO, 5%

Temperature: 37.0°C