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86D [127.5-9]
86D [127.5-9]
規(guī)格:
貨期:
編號:B163839
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 86D [127.5-9]
商品貨號 B163839
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for the sheep gamma/delta TCR and precipitates molecules of 42000 and 36000 daltons.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the sheep (ovine) gamma/delta T cell receptor
Cellular Products
immunoglobulin; monoclonal antibody; against the sheep (ovine) gamma/delta T cell receptor
Comments
Animals were immunized with double negative (DN) sheep T cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for the sheep gamma/delta TCR and precipitates molecules of 42000 and 36000 daltons.
Complete Growth Medium
CULTURE MEDIUM: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum,
10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgG1; kappa light chain
Name of Depositor CR Mackay
Deposited As mouse (B cell); mouse (myeloma)
References

Mackay CR, et al. Gamma/delta T cells express a unique surface molecule appearing late during thymic development. Eur. J. Immunol. 19: 1477-1483, 1989. PubMed: 2528463

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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