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Transfection Host:  These cells may be used for stable transfection studies.

immunoglobulin; IgM (surface)

It lacks a normal c-myc gene, but contains two copies of an ALV deregulated myc gene.

The cells retain the ability to rearrange the immunoglobulin light chain gene (IgL).

At the IgL locus, DT40 contains one rearranged and one germline allele.

The cell line exhibits a lymphoblastoid phenotype.

Infectivity assays revealed that DT40 releases low levels of infectious RAV-1.

Both the c-rel gene and the v-rel oncogenes induce major histocompatibility (MHC) class II antigen expression on the DT40 cell line. 

The expression of MHC class II is induced more rapidly by v-rel than c-rel and several weeks after infection, rel induced class II antigen as much as 50 fold more efficiently than c-rel. 

Hrdlickova R, et al. v-rel induces expression of three avian immunoregulatory surface receptors more efficiently than c-rel. J. Virol. 68: 308-319, 1994. PubMed: 8254742

Humphries EH, Zhang G. V-rel and C-rel modulate the expression of both bursal and non-bursal antigens on avian B-cell lymphomas. Curr. Top. Microbiol. Immunol. 182: 475-483, 1992. PubMed: 1490388

Baba TW, et al. Cell lines derived from avian lymphomas exhibit two distinct phenotypes. Virology 144: 139-151, 1985. PubMed: 2998040

Buerstedde JM, et al. Light chain gene conversion continues at high rate in an ALV-induced cell line. EMBO J. 9: 921-927, 1990. PubMed: 2155784

Kim S, et al. Ongoing diversification of the rearranged immunoglobulin light-chain gene in a bursal lymphoma cell line. Mol. Cell. Biol. 10: 3224-3231, 1990. PubMed: 2111450

Buerstedde JM, Takeda S. Increased ratio of targeted to random integration after transfection of chicken B cell lines. Cell 67: 179-188, 1991. PubMed: 1913816