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J558
J558
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貨期:
編號:B164878
品牌:Mingzhoubio

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產(chǎn)品名稱 J558
商品貨號 B164878
Organism Mus musculus, mouse
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease plasmacytoma; myeloma
Strain BALB/c
Applications
Tested and found negative for ectromelia virus (mousepox).
The cells are sensitive to dexamethasone and cortisol.
Storage Conditions liquid nitrogen vapor phase
Karyotype diploid
Derivation
The plasmacytoma was induced with mineral oil in a BALB/c mouse.
Antigen Expression
Antigen expression: H-2d; PC.1
Genes Expressed
immunoglobulin; monoclonal antibody; against alpha-1,3-dextran,H-2d; PC.1
Cellular Products
immunoglobulin; monoclonal antibody; against alpha-1,3-dextran
Comments
The cells are sensitive to dexamethasone and cortisol.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Isotype IgA; lambda light chain
Name of Depositor M Cohn
Deposited As Mus musculus
References

Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304

Gehring U, et al. Glucocorticoid action on hybrid clones derived from cultured myeloma and lymphoma cell lines. Proc. Natl. Acad. Sci. USA 69: 3124-3127, 1972. PubMed: 4343956

Halpern MS, Coffman RL. Polymer formation and J chain synthesis in mouse plasmacytomas. J. Immunol. 109: 674-680, 1972. PubMed: 5074326

Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881

Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714

Carson D, Weigert M. Immunochemical analysis of the cross-reacting idiotypes of mouse myeloma proteins with anti-dextran activity and normal anti-dextran antibody. Proc. Natl. Acad. Sci. USA 70: 235-239, 1973. PubMed: 4119224

Lundblad A, et al. Immunochemical studies on mouse myeloma proteins with specificity for dextran or for levan. Immunochemistry 9: 535-544, 1972. PubMed: 5036150

Cesari IM, Weigert M. Mouse lambda-chain sequences. Proc. Natl. Acad. Sci. USA 70: 2112-2116, 1973. PubMed: 4516208

Weigert MG, et al. Variability in the lambda light chain sequences of mouse antibody. Nature 228: 1045-1047, 1970. PubMed: 5483159

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