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KC-4G3 [KC-4(IgG3)]
KC-4G3 [KC-4(IgG3)]
規(guī)格:
貨期:
編號:B164905
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 KC-4G3 [KC-4(IgG3)]
商品貨號 B164905
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Adenocarcinoma
Applications
The antibody reacts with an antigen present on solid tumors (98 of 104 tested) and cross-reacts with minimal cross reaction with paraffin embedded (14 of 92) or frozen (1 of 33) normal tissues.
An antibody with the same specificity but of the IgM class is available (ATCC HB-8710).
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human carcinoma cells
Cellular Products
immunoglobulin; monoclonal antibody; against human carcinoma cells
Comments
Animals were immunized with prostatic adenocarcinoma cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with an antigen present on solid tumors (98 of 104 tested) and cross-reacts with minimal cross reaction with paraffin embedded (14 of 92) or frozen (1 of 33) normal tissues.
The antigen is found in the cytoplasm as a 490000 dalton molecule, and in the membrane and cytoplasm as a 438000 dalton molecule.
An antibody with the same specificity but of the IgM class is available (ATCC HB-8710).
Complete Growth Medium
Culture Medium: Modified Dulbecco's medium (see below)with 0.1 mM hypoxanthine
and 0.015 mM thymidine, 85%; fetal bovine serum, 15%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
fetal bovine serum, 90%; DMSO, 10%
Isotype IgG3
Name of Depositor Coulter Corporation
Deposited As mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Kortright KH, Hofheinz DE. Monoclonal antibody to a human carcinoma tumor associated antigen. US Patent 4,708,930 dated Nov 24 1987

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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