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KG-1
KG-1
規(guī)格:
貨期:
編號(hào):B164909
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) KG-1
商品貨號(hào) B164909
Organism Homo sapiens, human
Product Format frozen
Morphology lymphoblast-like
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease acute myelogenous leukemia
Age 59 years
Gender male
Ethnicity Caucasian
Applications
Responsive to colony stimulating activity (CSA).
Produces high levels of human leukocyte interferon upon induction by paramyxoviridae species.
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Clinical Data
male
Caucasian
59 years
Genes Expressed
human leukocyte interferon
Cellular Products
human leukocyte interferon
Comments
Responsive to colony stimulating activity (CSA).
Produces high levels of human leukocyte interferon upon induction by paramyxoviridae species.
See also ATCC CCL-246.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 10(5) viable cells/ml.
Interval: Maintain between 2 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile
Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19
Name of Depositor Genentech, Inc.
U.S. Patent Number
References

Koeffler HP, Golde DW. Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity. Science 200: 1153-1154, 1978. PubMed: 306682

Goeddel DV. Hybrid human leukocyte interferons. US Patent 4,678,751 dated Jul 7 1987

Cross References

Nucleotide (GenBank) : E00473 Human leukocyte interferon A (LeIFA) gene and its flanking sequence.

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于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
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