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KG-1a
KG-1a
規(guī)格:
貨期:
編號:B164910
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
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RNA

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產(chǎn)品名稱 KG-1a
商品貨號 B164910
Organism Homo sapiens, human
Tissue bone marrow
Cell Type promyeloblast, Macrophage
Product Format frozen
Morphology myeloblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease acute myelogenous leukemia
Age 59 years
Gender male
Ethnicity Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype The stemline chromosome number is 46 (pseudodiploid), with the 2S component occurring at 5.8%. Seven markers, including five ATCC CCL-246-specific markers, were found in most, if not all metaphases analyzed., Another marker ? del (7) was found only in about 50% of the metaphases. Normal chromosomes 5, 7, 8, 12, 17 and 22 were monosomic. The Y chromosome is detected in the Q-banded preparations.
Derivation
The variant subline KG-1a of the human acute myelogenous leukemia cell line KG-1 was isolated by H.P. Koeffler, et al.
Clinical Data
59 years
Caucasian
male
Comments

After the tenth passage, the parental KG-1 cells were cultured in two separate laboratories within the same department under identical conditions.

After 35 passages the cells in one laboratory expressed morphological differences from the parent line. The variant KG-1a was composed of undifferentiated promyeloblasts.

The cells did not stain for ASD chloroacetate esterase, alpha-naphthyl butyrate esterase or peroxidase.

Both populations exhibit many common characteristics. They share a similar doubling time, are negative for EBNA and VCA, express no surface immunoglobulins and exhibit identical HLA and isoenzyme profiles.

In contrast to the parental KG-1 (ATCC CCL-246) the KG-1a population is unresponsive to colony-stimulating factor in soft-agar culture and does not express the Ia-like antigen.

KG-1a cells are resistant to phorbol diester induced macrophage differentiation and proliferation of the cells is unaffected by the presence of phorbol diesters

.The KG-1a cells are morphologically, cytochemically, and functionally less mature than the parental KG-1.

Although derived from, and almost identical to, KG-1 (ATCC CCL-246) these cells do not spontaneously differentiate to granulocyte and macrophage like cells, do not express DR and do not respond to colony stimulating factor (CSF).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL.

Maintain cell density between 2 x 105 and 1 x 106 viable cells/mL.

Medium Renewal: Twice per week

Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19
Isoenzymes
AK-1, 0
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1-2
PGM3, 0
Name of Depositor DW Golde
Deposited As Homo sapiens
Passage History
After the tenth passage, the parental KG-1 cells were cultured in two separate laboratories within the same department under identical conditions.
After 35 passages the cells in one laboratory expressed morphological differences from the parent line. The variant KG-1a was composed of undifferentiated promyeloblasts.
References

Koeffler HP. Induction of differentiation of human acute myelogenous leukemia cells: therapeutic implications. Blood 62: 709-721, 1983. PubMed: 6192859

Koeffler HP, et al. An undifferentiated variant derived from the human acute myelogenous leukemia cell line (KG-1). Blood 56: 265-273, 1980. PubMed: 6967340

Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679

Chen H, et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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