| 產(chǎn)品名稱(chēng) |
Mo-B |
| 商品貨號(hào) |
B165182 |
| Organism |
Homo sapiens, human |
| Cell Type |
B lymphoblast |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
2 Cells Contain RETROVIRUS
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
hairy cell leukemia |
| Age |
33 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Applications |
transfection host |
| Clinical Data |
male Caucasian 33 years |
| Genes Expressed |
cells produce infectious HTLV II |
| Cellular Products |
cells produce infectious HTLV II |
| Comments |
The cells produce infectious human T cell leukemia virus and must be handled as a potential biohazard under P-2 conditions. The line is EBNA positive (EBNA+). |
| Complete Growth Medium |
Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
|
| Subculturing |
Protocol: The cells are subcultured every 7 days by centrifugation .For optimum results do not culture below 8 X 10(5 ) cells/ml or above 2 X 10(6) cells/ml. Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: none |
| STR Profile |
Amelogenin: X,Y CSF1PO: 10,13 D13S317: 9,13 D16S539: 11 D5S818: 11,12 D7S820: 9,12 THO1: 9.3 TPOX: 8,11 vWA: 17,18 |
| Isoenzymes |
ES-D, 1 G6PD, B GLO-I, 2 PGM1, 1 PGM3, 0 |
| Name of Depositor |
DW Golde |
| Deposited As |
Homo sapiens |
| References |
Chen IS, et al. Human T-cell leukemia virus type-II transforms normal human lymphocytes. Proc. Natl. Acad. Sci. USA 80: 7006-7009, 1983. PubMed: 6316341
Saxon A, et al. T-lymphocyte variant of hairy-cell leukemia. Ann. Intern. Med. 88: 323-326, 1978. PubMed: 305224
Chen IS, et al. Molecular characterization of genome of a novel human T-cell leukaemia virus. Nature 305: 502-505, 1983. PubMed: 6312323
|
