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Distribution host: Escherichia coli HB101 (ATCC 33694)

Size (kb): 6.3379998207092290

DESCRIPTION OF VECTOR:
Intact vector size: 6.338
Type of vector: plasmid
Cloning sites: SpeI BamHI SmaI EcoRI HindIII ClaI SalI XhoI
Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI
Construction: pRS426 (ATCC 77107), MET25 promoter
Host range: Candida robustaSaccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->, 200-1300
terminator: CYC1, ->, 2004-2264
MCS: XhoI...SpeI, ->, 2265-2322
promoter for expression: MET25, <-, 2339-2720
marker(s): ampR, <-, 3838-4768
replicon: 2 micron, ->, 4868-6208

Vector: p426 MET25 (plasmid)

Promoters: Promoter for expression MET25

Construction: pRS426 (ATCC 77107), MET25 promoter

Marker(s):URA3,ampR

Construct size (kb): 6.337999820709229

Features: marker(s): URA3
marker(s): ampR
promoter for expression: MET25
replicon: 2 micron
MCS: XhoI...SpeI
terminator: CYC1

YX-type (expression) shuttle vector

Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.8, 0.4; EcoRV--4.7, 1.7; PstI--4.5, 1.9.

Expression from the O-acetyl homoserine sulfhydrylase (MET25) promoter is repressed when cells are grown in the presence of methionine.

High copy number shuttle expression vector.

One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.

Temperature: 37.0°C

component of:ATCC 87670