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ET1c [lambda-EZZ-gamma3]
ET1c [lambda-EZZ-gamma3]
規(guī)格:
貨期:
編號:B244428
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 ET1c [lambda-EZZ-gamma3]
商品貨號 B244428
Designations ET1c [lambda-EZZ-gamma3]
Species Homo sapiens, human
Depositors KD Bloch
Applications
For Northern blots, the 1.2 or 0.9 kb EcoRI fragment is recommended.
Vector
Construct size (kb): 5.699999809265137
Insert
DNA: cDNA
Insert lengths(kb): 3.0
Tissue: umbilical vein endothelium, LPS-stimulated
Gene product: endothelin 1 [EDN1]
Alleles: A1, B1, B2, A2, B3, B4
Insert Size (kb) 3.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.2, 0.9, 0.9; HindIII--4.0, 1.7; SspI--2.3, 2.3, 0.7, 0.3; AvaI--4.3, 1.4; BglI--4.5, 1.2.
The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.75; EcoRI--1.2; SspI--1.79, 2.04.
Because of the internal EcoRI site, the insert cannot be excised intact. For Northern blots, the 1.2 or 0.9 kb EcoRI fragment is recommended.
This insert contains two identical copies of 3' 0.9 kb EcoRI fragments.
Enzyme(s) not detecting polymorphism: AvaII, BamHI, BanII, BclI, BglII, EcoRI, HindIII, MspI, NruI, PstI, SstI, StyII, XbaI, and XhoI (16 unrelated individuals).
References

Bloch KD, et al. Structural organization and chromosomal assignment of the gene encoding endothelin. J. Biol. Chem. 264: 10851-10857, 1989. PubMed: 2659594

Hoehe MR, et al. TaqI identifies a four allele DNA polymorphism of the human endothelin-1 gene (EDN1). Nucleic Acids Res. 19: 3161, 1991. PubMed: 2057379

Kenneth D Bloch, personal communication

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